During Ahmadinejad's government, along with the growing conflict between the goals of the Iran and the Israel in the region, especially in the wars of Israel with Hezbollah and Hamas, there has been an intensification of Iran-US. Hence, in view of the special relationship between the US and Israel on one hand and the growing conflict between the goals of the Iran and Israeli during the Ahmadinejad era, the role of the Israel as an important factor in intensifying the confrontation between Iran and the US can be evaluated. Accordingly, this article aims to examine the role of the Israel in the intensification of the opposition between the Iran and the United States during the Ahmadinejad’s presidency. This study (with a descriptive-analytical approach) concluded that: Israel has played an important role in increasing the aggressive policies of the US and, consequently, the escalation of Iran-US confrontation by: linking Ahmadinejad's nuclear program to his policies toward this regime, "securitizing" of Iran's nuclear and regional activities, presenting the idea of its “existential threat" to the international community and with "announced" and "action" policies and scenarios in line with the plan, imposing the threat of attack to Iran and following Iranophobia.